Development and Validation of a Capillary Electrophoresis Method for the Determination of Sulfate in Indinavir Sulfate Raw Material
نویسندگان
چکیده
O presente trabalho descreve um método simples e rápido por eletroforese capilar, utilizando detecção indireta (230 nm) para determinação quantitativa de sulfato em sulfato de indinavir (matéria-prima). Uma solução contendo 10 mmol L de molibdato de amônio e 0,15 mmol L de brometo de cetiltrimetilamônio (pH 7,5) foi utilizada como eletrólito de corrida. As amostras foram injetadas hidrodinamicamente com pressão 5000 Pa durante 3 s. A resposta foi linear no intervalo entre 10,1 μg mL a 79,8 μg mL. Os limites de detecção e quantificação foram de 0,34 e 1,13 μg mL, respectivamente. A precisão intra-dia, determinada como desvio padrão relativo, foi de 2,8% para dez preparações independentes da amostra. Os valores de recuperação para três diferentes níveis de concentração variaram de 96,9 a 102,4%. A metodologia foi aplicada a amostras reais. A concentração de sulfato na amostra variou de 13,3 a 13,5% (m/m, calculado com relação ao material seco).
منابع مشابه
Development and Validation of a Micellar Capillary Electrophoresis Method for Determination of IFNβ-1b in Lyophilized Formulation of a Biosimilar Product
Human interferons (IFNs) are key cytokines secreted by immune system. They have several effects such as antiviral and anti tumors activity, activating immune cells and healing of multiple sclerosis. The type IFNs present in humans are α, β and Υ. IFN β is a polypeptide, normally produced by fibroblasts and seems to be more species-specific than IFN . Structural properties of I FNs are importan...
متن کاملDevelopment and Validation of a Micellar Capillary Electrophoresis Method for Determination of IFNβ-1b in Lyophilized Formulation of a Biosimilar Product
Human interferons (IFNs) are key cytokines secreted by immune system. They have several effects such as antiviral and anti tumors activity, activating immune cells and healing of multiple sclerosis. The type IFNs present in humans are α, β and Υ. IFN β is a polypeptide, normally produced by fibroblasts and seems to be more species-specific than IFN . Structural properties of I FNs are importan...
متن کاملDevelopment and Validation of a Capillary Electrophoresis Method for Determining Dimethyl Sulfate, a Carcinogen; and Chloroacetyl Chloride a Potential Genotoxin at Trace Levels in Pharmaceuticals
متن کامل
Determination of Aciclovir and its Related Substance Guanine in Bulk Drug and Tablet Preparation by Capillary Electrophoresis
Two Capillary Zone electrophoresis (CZE) methods were developed in this study, one using an acidic buffer (sodium citrate pH = 2.5) and the other using a basic buffer (sodium tetraborate pH = 9.8). The two methods were compared on the basis of repeatability and reproducibility of results and the CZE method developed with the basic buffer was then selected for further studies. The method was ful...
متن کاملDevelopment and Validation of a HPLC Method for Determination of Pefloxacin in Tablet and Human Plasma
Objective(s) Developing and validating a simple, efficient, reproducible and economic reversed phase high performance liquid chromatographic (RP-HPLC) method for the quantitative determination of pefloxacin in bulk material, tablets and in human plasma. Materials and Methods A shim-pack CLC-ODS column and a mobile phase constituting acetonitrile: 0.025 M phosphoric acid solution (13:87 v/v, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006